Coding

Part:BBa_K1620002:Design

Designed by: Celio Dias Santos Jr   Group: iGEM15_UFSCar-Brasil   (2015-09-03)
Revision as of 20:00, 3 September 2015 by Celio.dias (Talk | contribs) (→‎Source)

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small heat shock protein IbpA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was made using original codon frequencies of E. coli.


Source

This part was designed from gene IbpA of E. coli K12 MG16555 genome, and synthesized by IDT. PCR amplifications were carried out using primers showed bellow and the 3A assembly method was made.

PCR primers:

Fwd: 5' - GAA TTC GCG GCC GCT TCT AGA GAT GCG TAA CTT TGA TTT ATC - 3'

Rev: 5' - TAC TAG TAG CGG CCG CTG CAG TTA GTT GAT TTC GAT ACG GC - 3'

References