Coding

Part:BBa_K1618024

Designed by: Mark Elms   Group: iGEM15_NRP-UEA-Norwich   (2015-09-03)
Revision as of 15:09, 3 September 2015 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Pro-GlgB-Rv3034c

Rv3034c is a gene which codes for a putative acyltransferase which acylates glycogen/starch chains. This enzyme is encoded with GlgB which codes for a branching enzyme which will cut alpha-1,4 links and reattach the cut glycogen strand and re-anneal the strand to the main glycogen compound through an alpha-1,6 linkage, therefore creating a branch. These enzymes are promoted by an IPTG-inducible promoter and are encoded within the pSB1C3 standard vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 283
    Illegal BamHI site found at 1491
    Illegal BamHI site found at 2644
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 917


[edit]
Categories
Parameters
None