Coding
BD-CRY2

Part:BBa_K1592005

Designed by: Shuyan Tang   Group: iGEM15_HUST-China   (2015-09-03)
Revision as of 10:14, 3 September 2015 by Registry (Talk | contribs)

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GalBD-CRY2 Fusion for Yeast-Two-Hybrid

The light-induced transcriptional control is based on a blue light stimulated photoreceptor, cryptochrome 2 (CRY2) and its interacting partner, CIB1. The CRY2/CIB1 interaction is entirely genetically encoded and does not require addition of any exogenous cofactors. The binding naturally reverses within minutes in the dark, allowing rapid shutoff of transcription by placing samples in the dark. This system is based on yeast two-hybrid interaction, in which a light-mediated protein interaction brings together two halves (a binding domain, GalBD and an activation domain, GAL4AD) of a split transcription factor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 849
    Illegal BglII site found at 1308
    Illegal BamHI site found at 1793
    Illegal XhoI site found at 218
    Illegal XhoI site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 733
    Illegal AgeI site found at 1462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137
    Illegal BsaI site found at 1085
    Illegal BsaI.rc site found at 494
    Illegal SapI.rc site found at 602


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