Coding

Part:BBa_K1699002

Designed by: Emil Ruvinov   Group: iGEM15_BGU_Israel   (2015-08-09)
Revision as of 08:28, 3 September 2015 by Shalevgo (Talk | contribs)

gRNA for SaCas9 targeting human ubiquitin B gene

Ribozyme flanked gRNA compatible for SaCas9. gRNA sequence complements 3 different loci in the second exon of human UBB gene. It has a hammerhead ribozyme on its 5' and an HDV ribozyme on its 3' end. Upon transcription the ribozymes should cleave the mRNA at specific locations to release the gRNA (6).

Usage and Biology

usage and biology


Characterization

characterization


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 146
    Illegal NgoMIV site found at 175
  • 1000
    COMPATIBLE WITH RFC[1000]



[edit]
Categories
//awards/part_collection/2015
Parameters
None