DNA

Part:BBa_K1732000:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-03)
Revision as of 07:39, 3 September 2015 by Registry (Talk | contribs)

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J23100-PelB-GlucHCO-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 546
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 115
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is codon optimized for E.coli. Sequence contains a PelB leader sequence that allows the Gaussia princeps luciferase proteins to secreted into the periplasmic space of the cell.


Source

Engineered from XXXXXXXX. Provided by the Bruchez lab at Carnegie Mellon.

References