Regulatory

Part:BBa_K1585115:Design

Designed by: Tobias Schwanemann   Group: iGEM15_Aachen   (2015-09-01)
Revision as of 20:15, 1 September 2015 by Tschwanemann (Talk | contribs) (→‎Source)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Anderson Promoter with lacI binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.

The part is shipped in backbone J61002 because the functionality is directly visible by adding IPTG. RFP (E1010) should get expressed then.

K15751xx design.png



Source

Assambly of J23115 and lacI-binding-site of R_0010

The "lacI binding site" of Part R_0010 differs from lacO from Part BBa_J33207.

References