Coding

Part:BBa_K1621006

Designed by: Ramona Emig, Rabea Jesser, Julika Neumann, Lara Stuehn   Group: iGEM15_Freiburg   (2015-08-28)
Revision as of 13:10, 28 August 2015 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Dihydroxyacid dehydratase (Salmonella Typhimurium)

This part contains the coding sequence of the enzyme dihydroxyacid dehydratase from //Salmonella typhimurium//. Its main biological function is the dehydratation of 2,3-dihydroxy-isovaleic acid into alpha-ketoisovaleric acid in the biosynthesis of isoleucine and valine. Additionally, the protein is immunogenic in humans as it was shown by the group of Prof. Dr. Michael Hust at TU Braunschweig (Meyer //et al.//, 2012). This is why the part can be used for diagnostic purposes concerning //S. typhimurium//. The sequence as well as an expression plasmid carrying its sequence in a His-tagged variant was obtained directly from this group.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None