Composite

Part:BBa_K1615001:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-08-27)
Revision as of 17:48, 27 August 2015 by Brooke (Talk | contribs) (→‎Design Notes)

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Morphine-6-dehydrogenase driven by LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2493
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 1648
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Morphine dehydrogenase is RFC25 compatible. Fusion was performed using RFC10 standard due to illegal NgoMIV site in F1 origin.

Source

Morphine dehydrogenase from Pseudemonas putida. Lac expression cassette from iGEM10_Washington.

References