Composite
Fla-Art175

Part:BBa_K1659001:Design

Designed by: Raphaella Hull   Group: iGEM15_Oxford   (2015-08-10)
Revision as of 10:09, 27 August 2015 by Weikongquee (Talk | contribs) (Created page with "__NOTOC__ <partinfo>BBa_K1659001 short</partinfo> <partinfo>BBa_K1659001 SequenceAndFeatures</partinfo> ===Design Notes=== ===Sources=== The artilysin Art-175 is a fus...")

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Artilysin Art-175 fused at N-terminal with flagellin 26-47 peptide segment



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 525
    Illegal AgeI site found at 724
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sources

The artilysin Art-175 is a fusion protein (SMAP-29 fused to the N-terminus of endolysin KZ144) created by Briers et al as laid out in their publication in 2014 [1]. Briefly, endolysin KZ144 originates from Pseudomonas aeruginosa bacteriophage varphiKZ. SMAP-29 originates from sheep genomic DNA.


References

[1] Briers, Y., Walmagh, M., Grymonprez, B., Biebl, M., Pirnay, J. P., Defraine, V., … Lavigne, R. (2014). Art-175 is a highly efficient antibacterial against multidrug-resistant strains and persisters of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 58(7), 3774–3784. http://doi.org/10.1128/AAC.02668-14