![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K1773022
Designed by: Sarunas Tumas Group: iGEM15_Vilnius-Lithuania (2015-08-27)
Cascade with pLux/cI right promoter and strong RBS
I-F type Cascade complex of I-F type CRISPR-Cas system. The Cascade complex is comprised of five proteins : Csy1, Csy2, Csy3 and Cas6f and an crRNA molecule. The Cascade complex distinguishes target DNA , and recruits Cas3 (BBa_K1773003) protein, which degrades the target DNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1891
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2408
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Categories
Parameters
None |