Part:BBa_K1755001

ribB coding sequence

ribB，the 3,4 dihydroxy-2-butanone-4-phosphate synthase。 We get this part from E.coli genome by PCR. It can increase the electric output by synthesis of riboflavin.

Besides, riboflavin itself is yellow. After the addition of ribB, the bacteria will be yellow.

Usage and Biology

The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.

This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.

Proteins known to be involved in this subpathway in this organism are: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)

This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.

Sequence

       10         20         30         40         50

MNQTLLSSFG TPFERVENAL AALREGRGVM VLDDEDRENE GDMIFPAETM

       60         70         80         90        100

TVEQMALTIR HGSGIVCLCI TEDRRKQLDL PMMVENNTSA YGTGFTVTIE

      110        120        130        140        150

AAEGVTTGVS AADRITTVRA AIADGAKPSD LNRPGHVFPL RAQAGGVLTR

      160        170        180        190        200

GGHTEATIDL MTLAGFKPAG VLCELTNDDG TMARAPECIE FANKHNMALV

      210 

TIEDLVAYRQ AHERKAS

Normal E.coli(left one)and E.coli with ribB(right one)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 387
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 277
  Illegal AgeI site found at 319
  
• 1000
  COMPATIBLE WITH RFC[1000]