Coding
tetA(C)f

Part:BBa_J31007:Design

Designed by: Sabriya Rosemond, Erin Zwack   Group: iGEM06_Davidson   (2006-07-12)
Revision as of 21:52, 6 December 2006 by Kahaynes (Talk | contribs) (→‎Design Notes)

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tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 290
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 316
    Illegal NgoMIV site found at 684
    Illegal NgoMIV site found at 844
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was amplified from pSB1AT3 and is identical to the Tet resistance gene found in pBR322. BBa_J31007 was cloned into the pSB1A2 plamsid.

This part was PCR amplified from pSB1AT3 using the following primers. The primers have non-annealing 5'- extensions that introduce an XbaI site to the left and a SpeI site to the right of the coding region. Primer annealing sites are shown in bold.
Forward: 5’ GCATTCTAG ATGAAATCTAACAATGCGCTCATC
Reverse: 5’ ATGCACTAG TTAGGTCGAGGTGGCCCGGC

Source

pSB1AT3

References