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Part:BBa_K1809024:Design

Designed by: David Morgan   Group: iGEM15_WPI-Worcester   (2015-08-26)
Revision as of 15:37, 26 August 2015 by Dmmorgan (Talk | contribs) (→‎Source)

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Const. Promoter-RBS-BrAFP-DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal AgeI site found at 163
    Illegal AgeI site found at 418
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used a strong promoter to produce high levels of expression of BrAFP.


Source

Composite of parts BBa_J23119 and BBa_K1809023

References