Generator

Part:BBa_K1773020

Designed by: Sarunas Tumas   Group: iGEM15_Vilnius-Lithuania   (2015-08-26)
Revision as of 09:52, 26 August 2015 by Registry (Talk | contribs)

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Cas3 gene with pLux/cI right promoter and Medium RBS

Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is an helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and Medium RBS site. In the I-F type CRISPR-Cas system Cascade complex distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2720
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1441
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2404
    Illegal AgeI site found at 2777
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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