Regulatory
hUPll

Part:BBa_K1722000

Designed by: Yin Xiao   Group: iGEM15_SZU_China   (2015-08-26)
Revision as of 07:53, 26 August 2015 by Registry (Talk | contribs)

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hUPll is a bladder tissue-specific promoter .

Uroplakin II (UPII) has been characterized as a bladder tissue-specific protein and the expression of uroplakin II was found to be limited to bladder-derived cells. 2015 SZU-iGEM use hUPII to drive the expression of the therapeutic genes(such as p21 and Bax) so that the gene is expressed only in bladder cells and systematic toxicity is minimized. We tested hUPII gene in HFC(Human Fiber Epithelial Cells)，5637，T24 and Hela cell lines and found it can confer preferential expression of genes in the bladder urothelium like HFC, which are normal bladder cells, 5637 and T24, which are bladder cancer cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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