Composite

Part:BBa_K1800000:Design

Designed by: Laura Lee Irvin   Group: iGEM15_Georgia_State   (2015-08-25)
Revision as of 00:21, 26 August 2015 by Lirvie (Talk | contribs) (→‎Source)


Mambalgin-1 for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the design, we used assembly standard 10 and also included a start codon in the Mambalgin-1 cDNA sequence and had it synthesized with an RBS region, lacI promoter region, MYC epitome, and 6xHis tag for optimized expression in E. coli.


Source

Mambalgin-1 sequence (BBa_k1110003), RBS (BBa_j61101), lacI Promoter (BBa_R0011), MYC and 6xHis tag was copied from the pGAPZα vector sequence.

This was synthesized by IDT.

References