Regulatory

Part:BBa_K1585101:Design

Designed by: Tobias Schwanemann   Group: iGEM15_Aachen   (2015-08-24)
Revision as of 17:01, 24 August 2015 by Tschwanemann (Talk | contribs) (→‎Design Notes)


Anderson Promoter with lacI binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The "lacI binding site" of Part R_0010 differs from lacO from Part BBa_J33207 although they should be same. We choosed part BBa_R0010 because we assumed older parts would be more reliable.

Source

Assambly of J23100 and lacI-binding-site of R_0010

References