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Part:BBa_K1758120:Design

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-13)
Revision as of 23:39, 21 August 2015 by Mrfreeze (Talk | contribs) (→‎Design Notes)


RNase E regulating protein RraA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amplification with Gibson-primer:

  • Amplification of regulator of ribonuclease activity A (rraA):

Forward primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#RraA_fwd RraA_fwd]


Reverse primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#RraA_rev RraA_rev]


  • Amplification of the pSB1C3-Backbone:

Forward primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_pre pSB1C3_RraA_pre]


Reverse primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_suf pSB1C3_RraA_suf]


Ligation via [http://2015.igem.org/Team:Bielefeld-CeBiTec/Protocols Gibson-Assembly].

Source

RraA was obtained via PCR on E. coli K12 genomic DNA.

References