Composite

Part:BBa_K1604022:Design

Designed by: Veronica Pinamonti   Group: iGEM15_UNITN-Trento   (2015-08-20)
Revision as of 19:37, 20 August 2015 by Registry (Talk | contribs)

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araC-pBAD + β-carotene + J23100 + blh


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
    Illegal NheI site found at 32
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2041
    Illegal BamHI site found at 4082
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
    Illegal NgoMIV site found at 3618
    Illegal NgoMIV site found at 3748
    Illegal AgeI site found at 1876
    Illegal AgeI site found at 2833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1858


Design Notes

The two parts was joined together by strandard assembly, before it beta-carotene was amplified by Phusion PCR.


Source

AraC-pBAD promoter comes from E.coli genome, while ctrEIBY are from Pantoea ananatis (formerly Erwinia uredovora) genome. Blh gene and its promoter are synthetized and codon optimezed by GeneScript.

References