Part:BBa_K310004:Experience

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Stockholm 2015 iGEM Team

This part doesn’t work. The part is not the described MicF target, rather it appears to be a Cu-responsive transcriptional regulator from Salmonella enterica.

Restriction analysis with EcoRI and PstI shows an insert that is much larger than the expected 190 bp.

We therefore sequenced the part and found that it is a 684 bp BioBrick flanked by the BioBrick prefix and suffix. BLAST alignment of the part showed that it probably is a Cu-responsive transcriptional regulator from Salmonella enterica.

Left: restriction analysis gel of K310004 in pSB1C3, digested with EcoRI and PstI.

Middle: Simulated gel (SnapGene) with K310004 sequence provided by iGEM Team UIUC Illinois 2010.

Right: Simulated gel (SnapGene) with K310004 sequence provided by iGEM Team Stockholm 2015

>BBa_K310004 Part-only sequence (684 bp) AAAGAGGAGAAATACTAGATGCAGTTCCATATTGATGACATGACCTGCGGCGGCTGCGCCAGTACGGTAAAAAAGACGATTCTGACTCTCGA TGCTAATGCGACGGTGAGAACTGACCCGGCGACGCGTCTGGTTGACGTTGAAACGTCGCTATCCGCGGAGCAGATTGCCGCCGCCCTGCAA AAGGCCGGTTTCCCGCCGCGCGAGACCTAATACTAGATGAACATCGGTAAAGCAGCTAAAGCATCGAAAGTCTCGGCCAAAATGATTCGCTA CTATGAACAGATTGGTCTGATTCCCGCGGCAAGTCGGACGGATTCCGGCTATCGGGCCTATACCCAGGCTGATGTTAATCAATTGCATTTTAT ACGCCGCGCGCGCGACCTCGGTTTTTCAGTTGCTGAAATCAGCGACTTACTGAATCTTTGGAATAACCAGTCGCGGCAAAGCGCTGACGTCA AACGCCTGGCGCAGACGCACATTGATGAACTGGACAGACGTATCCAGAACATGCAGCACATGGCGCAAACCCTCAAAGCGCTGATTCACTG CTGCGCCGGCGACGCGCTGCCAGATTGCCCCATTCTGCATACGCTTGGA

User Reviews

UNIQ480585df9004c533-partinfo-00000000-QINU

Stockholm 2015

This part is not the MicF target.

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