Composite

Part:BBa_K1604020

Designed by: Elisa Godino   Group: iGEM15_UNITN-Trento   (2015-08-18)
Revision as of 17:44, 18 August 2015 by Registry (Talk | contribs)

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araC-pBAD + beta-carotene

This Composite Biobrick is created by standard assembly of Part BBa_K731201 and Part BBa_K274200, which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1C3 (high copy, Chloramphenicol resistance). BBa_K274200 contains the enzymes that convert colourless farnesyl pyrophosphate to orange beta-carotene. The carotene operon contains CrtE, CrtB, CrtI and CrtY each one with individual ribosome binding site. This Composite Biobrick has been put under inducible araC-pBAD promoter that is positively regulated by arabinose.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2721
    Illegal NgoMIV site found at 2851
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1936
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
n/aaraC-pBAD + beta-carotene