Part:BBa_K1790000:Design
biosensors detect food allergens. A proteins conformation change in response to ligand binding coupl
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 990
Illegal BglII site found at 1068
Illegal XhoI site found at 67 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 316
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1017
Design Notes
http://imgur.com/PtWnsjU,jz7cmP7,EKg1L2N#0 http://imgur.com/PtWnsjU,jz7cmP7,EKg1L2N#1 http://imgur.com/PtWnsjU,jz7cmP7,EKg1L2N#2
Source
E.Coli - E. coli bacteria were discovered in the human colon in 1885 by German bacteriologist Theodor Escherich. E. coli is often referred to as the best or most-studied free-living organism. More than 700 serotypes of E. coli have been identified. The E. coli that are responsible for the numerous reports of contaminated foods and beverages are those that produce Shiga toxin, so called because the toxin is virtually identical to that produced by Shigella dysenteria type 1 The bacterium can be grown and cultured easily and inexpensively in a laboratory setting, and has been intensively investigated for over 60 years. E. coli is the most widely studied prokaryotic model organism, and an important species in the fields of biotechnology and microbiology, where it has served as the host organism for the majority of work with recombinant DNA E. coli is a Gram-negative (bacteria which do not retain crystal violet dye), facultative anaerobic (that makes ATP by aerobic respiration if oxygen is present, but is capable of switching to fermentation or anaerobic respiration if oxygen is absent) and nonsporulating bacteria.
References
T7 system
http://www.genomics.agilent.com/article.jsp?pageId=472