![](https://parts.igem.org/images/partbypart/icon_reporter.png)
Part:BBa_K1758101
Translation enhancing 5-UTR + sfGFP
This part includes a translation enhancing sequence, 5'-UTR, in front of sfGFP coding sequence (BBa_I746916). It also contains a double terminator made of <a href"https://parts.igem.org/wiki/index.php?title=Part:BBa_B0010">B0010</a> and <a href"https://parts.igem.org/wiki/index.php?title=Part:BBa_B0012">B0012</a>. The translation enhancing sequence improves the binding of the mRNA to the ribosome (<a href="http://www.ncbi.nlm.nih.gov/pubmed/2676996">Olins et al. 1989</a>) and is especially helpful in in vitro cell free protein synthesis. The sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency (<a href="http://www.ncbi.nlm.nih.gov/pubmed/23927491">Takahashi et al. 2013</a>).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 59
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