Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Coding

Part:BBa_K1758120:Design

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-13)
Revision as of 22:13, 13 August 2015 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


RNase E regulating protein RraA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amplification with Gibson-primer:

  • Amplification of regulator of ribonuclease activity A (RraA):

Forward primer RraA

5'-ATGAAATACGATACTTCCGAGCTTTG-3'

Reverse primer RraA

5'-TCATTCAATATCCAGCGGATCTTC-3'

  • Amplification of the pSB1C3-Backbone:

Forward primer pSB1C3 RraA(43 bp)

5'-TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG-3'

Reverse primer pSB1C3 RraA(43 bp)

5'-TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC-3'

Ligation via [http://2015.igem.org/Team:Bielefeld-CeBiTec/Protocols Gibson-Assembly].



Source

RraA was obtained via PCR on E. coli K12 genomic DNA.

References