Regulatory
R0011-N

Part:BBa_K1725080

Designed by: Mhairi Davidson   Group: iGEM15_Glasgow   (2015-08-11)
Revision as of 15:43, 11 August 2015 by Registry (Talk | contribs)

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Promoter (lacI regulated, lambda pL hybrid) with extra Nhe I site

Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be: repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]). induced by IPTG in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range

Extra Nhe1 site added, for ease of checking for BioBrick with a digest

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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