Coding

Part:BBa_K1723000:Design

Designed by: Gregoire Thouvenin   Group: iGEM15_EPF_Lausanne   (2015-08-10)
Revision as of 16:07, 10 August 2015 by Registry (Talk | contribs)

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dCas9-ω


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
    Illegal BamHI site found at 4212
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original dCas9-ω given to us by the Bikard lab contains an EcoRI restriction site. We performed a site-directed mutagenesis to remove it.


Source

A plasmid containing dCas9_ω (pdCas9) was given to our iGEM team by David Bikard.

References