Project

Part:BBa_K1790003:Design

Designed by: einav bar hanin   Group: iGEM15_Danzi_Kesh_8   (2015-08-04)
Revision as of 07:35, 10 August 2015 by Or (Talk | contribs)


The GlnHPQ high-affinity glutamine transport system is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95]. Based on sequence similarity, GlnH is the periplasmic glutamine-binding protein, GlnQ is the ATP-binding component, and GlnP is the membrane component of the ABC transporter. Mutation of glnP results in the impaired ability to transport glutamine as well as the inability to utilized glutamine as a sole source of carbon [Masters81, Nohno86]. Expression of the cloned glnHPQ genes on a plasmid vector restored the glnH, glnP and glnQ mutants' abilities to transport glutamine and utilize glutamine as a sole carbon source [Nohno86].






T7 system

http://www.genomics.agilent.com/article.jsp?pageId=472

HAD

http://www.biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=G6932