Composite

Part:BBa_K1582020

Designed by: LIU JIASHU   Group: iGEM15_Tianjin   (2015-08-07)
Revision as of 15:59, 7 August 2015 by Registry (Talk | contribs)

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FsC+sJanus Fusion Protein

Cutinase, which hydrolyses cutin, has previously shown that it can also hydrolyze plastic, such as poly(ethylene terephthalate) (PET). Hydrophobin, small secreted fungal protein, has previously shown that it can improve the rate of plastic hydrolysis. We choose cutinase from Fusarium solani (FsC) to investigate its activity of hydrolyzing PET and whether its PET hydrolyzing activity could be enhanced by fusion to class Ⅱ Hydrophobin HFBI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 907
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 301
    Illegal SapI site found at 916


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Categories
Parameters
None