Part:BBa_K1582005

GFP+sJanus-m Fusion Protein

HFBI is a modle of class I of hydrophobins, which are naturally produced by filamentous fungi. We use serine, which is similar with cysteine in structure, to replace all of its conserved eight-cysteine residues and get the mutant of HFBI. Hydrophobins are amphipathic, which means they have the hydrophobic part and hydropholic part at the same time. HFBI is a kind of hydrophobins and it has magic properties. We combine GFP with HFBI by a linker and make them become a fusion protein. This fusion protein could be used in aqueous two-phase systems to make itself seperated frome mixture. Meanwile, hydrophobin has a property that it can generate a membrane on the surface of solid automatically, which is called self-assembly. We can get a fluorescent protein' membrane by it.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 976
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 644
  Illegal SapI site found at 985