Composite

Part:BBa_K1648003

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-07-17)
Revision as of 06:54, 17 July 2015 by Registry (Talk | contribs)

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Recombination Template for mamXY,mamGC and mms Operons with Promotor and Terminator

Consist of six fragments from magnetosome island of Magnetospirillum gryphiswaldense genome.

Due to potential difficulty in introducing the large magnetosome formation operons into Azotobacter vinelandii, a "template" consists of flanking sequences from mamAB operonsexcept the mamO operon is designed for homologous recombination.

Part containing this template (K1648XXX) together with another part with mamO operon(K1648XXX) is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1413
    Illegal BamHI site found at 512
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 683
    Illegal NgoMIV site found at 865
    Illegal NgoMIV site found at 955
    Illegal NgoMIV site found at 1363
    Illegal AgeI site found at 566
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 550
    Illegal BsaI site found at 649
    Illegal BsaI site found at 930


[edit]
Categories
Parameters
n/aRecombination Template for mamXY,mamGC and mms Operons with Promotor and Terminator