DNA

Part:BBa_K1648001

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-07-14)
Revision as of 06:46, 17 July 2015 by S1155046866 (Talk | contribs)

Construct Map for K1648001

Recombination Template for mamXY, mamGC and mms Operons

Consist of six fragments from magnetosome island of Magnetospirillum gryphiswaldense genome.

Due to potential difficulty in introducing the large magnetosome formation operons into Azotobacter vinelandii, a "template" consists of flanking sequences from mamXY, mamGFDC and mms6 operons is designed for homologous recombination.

Part containing this template (K1648002) is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1333
    Illegal BamHI site found at 432
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 603
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 875
    Illegal NgoMIV site found at 1283
    Illegal AgeI site found at 486
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 470
    Illegal BsaI site found at 569
    Illegal BsaI site found at 850


[edit]
Categories
Parameters
n/aRecombination Template for mamXY, mamGC and mms Operons