Coding

Part:BBa_K1763000:Design

Designed by: Daniel Cancilla   Group: iGEM15_UCLA   (2015-05-21)
Revision as of 01:08, 17 July 2015 by Dcancilla (Talk | contribs) (→‎Source)

Apis mellifera (honeybee) silk fibroin 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • [http://www.sciencedirect.com/science/article/pii/S0142961209013805 This paper] referred us to the some of the honeybee silk sequences. There are 4 different honeybee silk sequences in native silk that come together to form a silk fiber. However, it was shown that by just expressing one of the three sequences, you could obtain silk with comparable physical properties to the native honeybee silk (Weisman). So we chose Apis mellifera silk fibroin 3 and we synthesized the sequence, making sure to omit the signal peptide at the 5' end of the protein.

Source

  1. Apis mellifera genome. Reference Genbank FJ235090.1
  2. Weisman, Sarah, et al. "Honeybee silk: Recombinant protein production, assembly and fiber spinning." Biomaterials 31.9 (2010): 2695-2700.
  3. Sutherland, Tara D., et al. "Single honeybee silk protein mimics properties of multi-protein silk." PLoS One 6.2 (2011): 16489.

APA

References