DNA

Part:BBa_K1648000:Design

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-07-07)
Revision as of 02:04, 15 July 2015 by Kpsleung1990 (Talk | contribs) (→‎Design Notes)

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Recombination Template for mamAB Operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 291
    Illegal AgeI site found at 810
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 536


Design Notes

In order to form a homologous recombination template, we selected 250 bp of 5' and 3' ends from mamHIEJKLMN and mamOPAQRBSTU (parts of mamAB operon) as flanking sequences. Fragments are joined via overlapping PCR.

K1648000 History.png

Source

Magnetospirillum gryphiswaldense MSR-1 v2, complete genome

References