DNA

Part:BBa_K1648000

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-07-07)
Revision as of 16:05, 14 July 2015 by S1155046866 (Talk | contribs)

Recombination Template for mamAB Operon

Construct Map for K1648000

This part is constructed by four fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of mamAB(a part of genome from magnetospirillum gryphiswaldense magnetosome island).



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 291
    Illegal AgeI site found at 810
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 536


[edit]
Categories
Parameters
n/aRecombination Template for mamAB Operon