Part:BBa_K1075047

pDawn-RBS32-ccdB-TT

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

This part is a combination of the light-inducible BBa_K1075044 and the toxin ccdB BBa_K1075029.

Usage and Biology

pDawn-RBS32-ccdB-TT can be used to photocontrol the expression of the toxin ccdB. In this system pDawn (BBa_K1075044) can be regulated by blue light, while ccdB (BBa_K1075029) acts as a killswitch.

The ccd operon of the F plasmid encodes CcdB, a toxin targeting the essential gyrase of Escherichia coli, and CcdA, the unstable antidote that interacts with CcdB to neutralize its toxicity.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2001.02492.x/abstract;jsessionid=6CE0056B73608A461539FF28D894B906.f03t01]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2171
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 63
  Illegal NgoMIV site found at 195
  Illegal NgoMIV site found at 289
  Illegal NgoMIV site found at 582
  Illegal NgoMIV site found at 1076
  Illegal NgoMIV site found at 1094
  Illegal NgoMIV site found at 1184
  Illegal AgeI site found at 414
  Illegal AgeI site found at 1542
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1643
  Illegal BsaI site found at 3659
  Illegal BsaI.rc site found at 525