Translational_Unit

Part:BBa_K1033120

Designed by: Magnus Bergman, Pontus Danielsson, Alexander Wirtanen, Nils Anlind, Christoffer Frisk, Thorsteinn Olaufsson, Malin Borg, Niclas Sandberg   Group: iGEM13_Uppsala   (2013-09-02)
Revision as of 14:55, 11 June 2015 by Vinoo (Talk | contribs) (→‎Registry Curation)

B0034-ExpTag-Linker-Miraculin-Linker-6xHis

Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum".

This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.

Applications

Miraculin can be used as a sweetener.


Characterization data from Uppsala iGEM 2013

We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.


Registry Curation

Original sequence changed by iGEM HQ

  • Change - "TAT" added to start of part sequence, notified by Team [http://2015.igem.org/Team:UMaryland UMaryland 2015 Team]
    • Reason - To reflect sample sequence
  • Change - Part type from "Coding" to "Translational Unit"
    • Reason - RBS in front of coding Region

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None