Device

Part:BBa_J119386:Design

Designed by: Julia Preziosi, Jon Trocosso   Group: Eckdahl Lab   (2015-06-09)
Revision as of 15:08, 9 June 2015 by Registry (Talk | contribs)

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tCloneTetRed; Fusion Protein Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1094
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1240
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1266
    Illegal NgoMIV site found at 1634
    Illegal NgoMIV site found at 1794
    Illegal AgeI site found at 2741
    Illegal AgeI site found at 2853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849
    Illegal BsaI.rc site found at 42


Design Notes

It was unknown whether the Tet+Red fusion protein would work as well as Tet+GFP did.


Source

The part comes from J119361 tCloneRed and J119140 TetA. The linker sequence was developed from K598018 tetA+GFP fused protein by Qingyang XIAO.

References