Device

Part:BBa_J100210

Designed by: Monica Prudencio   Group: Campbell M Lab   (2015-06-02)
Revision as of 20:00, 2 June 2015 by Registry (Talk | contribs)

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tCloneRed with RS4 Transcriptional Riboswitch

Using Golden Gate Assembly, the transcriptional riboswitch RS4 (from Wachsmuth, et al.) was ligated into the destination vector tClone Red (J119361) in place of the insert (between BsaI sites). This riboswitch is controlled by theophylline, allowing transcription if theophylline is present. However, it also has shown increased transcription levels in the presence of caffeine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 761
    Illegal AgeI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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