Composite

Part:BBa_K1648050

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-05-31)
Revision as of 08:02, 31 May 2015 by Registry (Talk | contribs)

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PfOprF

nifA from Azotobacter vinelandii, with mutation L120P. Constitutive active form of nifA transcriptional activator, leading to constitutive expression of nitrogenase regardless presence of nitrogen source.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 844
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1236
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 757


[edit]
Categories
Parameters
n/aPfOprF