Coding
AvOprF

Part:BBa_K1648045:Design

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-05-21)
Revision as of 07:24, 31 May 2015 by Kpsleung1990 (Talk | contribs) (→‎Source)


AvOprF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 475


Design Notes

PCR with BBa_K1314016 as template using the following primers:

>SH3 Domain F
GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCAGAGTATGTGCGGGC

>SH3 Domain R
GTTTCTTCCTGCAGCGGCCGCTACTAGTATACTTCTCCACGTAAGGGACAGG

Source

BBa_K1314016

References