Coding
AvOprF

Part:BBa_K1648045:Design

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-05-21)
Revision as of 07:24, 31 May 2015 by Kpsleung1990 (Talk | contribs) (→‎Design Notes)


Recombination Template for mamAB Operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 291
    Illegal AgeI site found at 810
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 536


Design Notes

PCR with BBa_K1314016 as template using the following primers:

>SH3 Domain F
GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCAGAGTATGTGCGGGC

>SH3 Domain R
GTTTCTTCCTGCAGCGGCCGCTACTAGTATACTTCTCCACGTAAGGGACAGG

Source

Synthesis.

References