Reporter
Part:BBa_J61032
Designed by: John Anderson Group: Arkin Lab (2006-11-10)
Revision as of 21:11, 10 November 2006 by JCAnderson (Talk | contribs)
Alkaline phosphatase [phoA]
Alkaline phosphatase coding sequence (no rbs, promoter, or terminator present) from Citrobacter. This bacterium's phoA was chosen due simply to the absense of Biobrick restriction sites. There may be some neutral point mutations relative to the stated sequence, but the construct is fully functional. This part exists in plasmid pSB1A2 (pSB1A2-Bca1032) and in J61003 (pBca1020-Bca1032) under the control of a Tet promoter and RBS b0034.
Alkaline phosphatase is used to report gene expression activity. When expressed in the cytoplasm, PhoA can be assayed by a PNP (para-nitrophenol phosphate) hydrolysis assay--an assay very similar to a standard Miller Assay for LacZ activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 310
Illegal NgoMIV site found at 757 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/enzyme
//function/reporter/color
//function/reporter/color
None |