Regulatory

Part:BBa_J119375:Design

Designed by: Anthony Eckdahl   Group: Eckdahl Lab   (2015-02-26)
Revision as of 13:35, 27 March 2015 by Eckdahl (Talk | contribs) (→‎Design Notes)


81 Mutated Ptac Promoters with Varying Strengths


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutations were introduced into the Ptac promoter using NNNNNN in place of the TTGACA of the -35 region. Synthetic oligonucleotides carry the 4096 possible sequences were cloned via Golden Gate Assembly into pClone Red.

Source

Synthetic oligonucleotides cloned with BsaI Golden Gate Assembly into pClone Red (BBa_J119137).

References