Regulatory

Part:BBa_J119377:Design

Designed by: Anthony Eckdahl   Group: Eckdahl Lab   (2015-03-27)
Revision as of 13:34, 27 March 2015 by Eckdahl (Talk | contribs) (→‎References)

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Psimp2 Promoter with Four Consensus Variants


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The consensus sequence from De Mey et al. shown below was used to generate a promoter that has a simple sequence, by using W=T, N=C, R=A, and D=C, but avoiding runs of 4 or more Gs or Cs. The resulting promoter was named Psimp1.

De Mey 2007 Figure 1.jpg

Source

Synthetic oligonucleotides cloned with BsaI Golden Gate Assembly into pClone Red (BBa_J119137).

References

De Mey M, Maertens J, Lequeux GJ, Soetaiert WK, Vandamme EJ (2007) Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering. MC Biotechnol 7:34.