Regulatory

Part:BBa_J119375

Designed by: Anthony Eckdahl   Group: Eckdahl Lab   (2015-02-26)
Revision as of 02:35, 27 February 2015 by Eckdahl (Talk | contribs)

81 Mutated Ptac Promoters with Varying Strengths

Mutations were introduced into the Ptac promoter using NNNNNN in place of the TTGACA of the -35 region. The strength of each of 83 mutant promoters was measured by cloning them into pClone Red (J119137) and measuring RFP expression with fluorometry.

83 Ptac promoters.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None