Part:BBa_M36900:Experience

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Barcode # (glycerol stock): 133018572

Plasmid Name: NeginandAbbey

Antibiotic Resistance: Ampicillin

DNA 2.0 Gene #: 193570

Organism Expressed in HeLa

Sensor

In experimentation of this part's performance, our goals were to establish whether or not the device at hand would perform as a functional salt sensor in eukaryotic HeLa cells, by quantifying fluorescence level outputs actuated by a coupled actuator for various levels of salt stress input, both in terms of stress duration and NaCl concentration in the solution. At all experimental conditions (40,80,120 micromolar NaCl solution x 35, 70 min exposure time) our transformed HeLa cells exhibited significantly larger levels of fluorescence after salt stress exposure, as compared to the mock-transfected cells exposed to identical environment. However,

Please see the attached .pdf for details on device performance as a dose response curve over two ranges of mercury ion concentration. In the graphs, red bars represent growth of cells with the BBa_M36567 construct and blue bars represent growth of normal E.coli K12 cells with no mercury reduction plasmid

User Reviews

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