Composite

Part:BBa_J100205

Designed by: Monica Prudencio   Group: Campbell M Lab   (2014-12-07)
Revision as of 17:52, 7 December 2014 by Moprudencio (Talk | contribs)

repClone Red

We designed repClone Red to test the function of repressors. Internal BsaI sites allow the insertion of the pTet promoter to activate TetR. Variations on this promoter or introduction of tetracycline will affect the activity of the repressor, as indicated by the fluorescence of colonies containing the plasmid. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 9
    Illegal AgeI site found at 2288
    Illegal AgeI site found at 2400
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1638
    Illegal BsaI.rc site found at 1517


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