Composite

Part:BBa_M36921

Designed by: Leah Chase   Group: Stanford BIOE44 - S11   (2014-10-22)
Revision as of 06:22, 5 December 2014 by Lmchase (Talk | contribs)

Zinc Sensor

This composite part contains a constitutive promoter, ribosome-binding-site, a gene for ZntR, an E. Coli terminator, and a promoter that is ZntR regulated. In the absence of Zinc ions, the ZntR protein binds to the ZntR promoter binding site. In the presence of zinc ions, ZntR undergoes a conformation, does not attach to the binding site, and allows for transcription of our actuator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 194
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 194
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 190
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 194
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/sensor/light
Parameters
emissionComet Fluorescence (wavelength 403 nm : 511 nm)
resistanceKanamycin