![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_M36921
Designed by: Leah Chase Group: Stanford BIOE44 - S11 (2014-10-22)
Zinc Sensor
This composite part contains a constitutive promoter, ribosome-binding-site, a gene for ZntR, an E. Coli terminator, and a promoter that is ZntR regulated. In the absence of Zinc ions, the ZntR protein binds to the ZntR promoter binding site. In the presence of zinc ions, ZntR undergoes a conformation, does not attach to the binding site, and allows for transcription of our actuator.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 194
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 194 - 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 194
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 194
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//function/sensor/light
emission | Comet Fluorescence (wavelength 403 nm : 511 nm) |
resistance | Kanamycin |