Composite

Part:BBa_M36938:Experience

Designed by: Emily Kelly   Group: Stanford BIOE44 - S11   (2014-10-23)
Revision as of 06:59, 4 December 2014 by CME2 (Talk | contribs)

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Applications of BBa_M36938

Stanford Location:

Box Name: BIOE44 S14

Glycerol Freezer Stocks:

Barcode# :133011796, Plasmid Name: CME LCPUFA_Dasher, Resistance Type: amp, DNA 2.0 Gene #:193425, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode# : 133024687, Plasmid Name: CME LCPUFA_Dasher, Resistance Type: amp, DNA 2.0 Gene #:193425, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode# : 133010087, Plasmid Name: CME LCPUFA_Dasher, Resistance Type: amp, DNA 2.0 Gene #:193425, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode# : 133011361, Plasmid Name: CME LCPUFA_Dasher, Resistance Type: amp, DNA 2.0 Gene #:193425, Organism: E. coli, Device Type: LCPUFA Actuator.

Performance Data:

Protein Presence Test: GFP Fluorescence Reading

GFPchart.gif


GFPgraph.gif


Conclusion: The target protein (enoyl-[acyl-carrier-protein] reductase) is synthesized within the E. coli cells. This result is statistically significant.


Protein Functionality Test: pH Reading

Functional (enoyl-[acyl-carrier-protein] reductase) is expected to partake in a naturally occurring reaction within the E. coli cell. This reaction consumes hydrogen ions. Thus a functional protein is expected to increase the overall pH of the cell.

Note: pH Readings were not taken for this composite part but for its sister part BBa_M36939 (lacks Comet GFP). We suspect that similar results would be obtained for this construct (containing Comet GFP). This data is included simply to provide a full analysis of the mechanism and its potential.

Standard(no GFP) pH Readings: 6.91 6.64 6.49 6.42 6.64 6.45 6.34 6.4

Control pH Readings:

6.56 6.49 6.42 6.36 6.25 6.26 6.29 6.34

Standard Average pH: 6.53625

Control Average pH: 6.37125

p=0.011066469 ---> p < 0.05

Conclusion: Production of the target protein (enoyl-[acyl-carrier-protein] reductase) causes an increase in pH, as is to be expected. Therefore we conclude that the target protein is functional. This is statistically significant.

User Reviews

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